Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter HEK 293 GPR150 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0375C1A)
The PathHunter GPR150 (AXOR70) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR150 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR150 and EA-tagged β-Arrestin-2. Activation of GPR150 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS MC3R β-Arrestin Cell Line, 2 vials. (93-0984C3)
The PathHunter MC3R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC3R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC3R and EA-tagged β-Arrestin-2. Activation of MC3R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter LTB4R Total Internalization U2OS Cell Line, 2 vials. (93-0810C3)
The PathHunter LTB4R Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of LTB4R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged LTB4R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the LTB4R receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Human respiratory; syncytial virus; Strain: A2 Purified
Human respiratory syncytial virus, Purified strain A2 is propagated in HeLa cells (ATCC CCL-2). This strain was isolated in 1961 from the lower respiratory tract of an infant with bronchiolitis and bronchopneumonia in Melbourne, Australia. This product is provided as a purified preparation and has applications in respiratory disease research and assay development.
Eurofins Discoverx ChemiScreen D5 Dopamine Receptor Membrane Preparation, 1 vial. (HTS129M)
The ChemiScreen D5 Dopamine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of DRD5 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PathHunter CHO-K1 CX3CR1 β-Arrestin Cell Line, 2 vials. (93-0956C2)
The PathHunter CX3CR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CX3CL1/fractalkine) based activation of CX3CR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CX3CR1 and EA-tagged β-Arrestin-2. Activation of CX3CR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Pfizer Pneumococcal polysaccharide powder Type 6 (d6A) 200mg
Purified pneumococcal polysaccharide type 6A (US Type 6) is manufactured by Pfizer, Inc., for small-quantity distribution by ATCC. The pneumococcal powder type 6A is a component of the current 13-valent vaccine against Streptococcus pneumoniae. The pneumococcal polysaccharide type 6A can be used to assay for type-specific antibodies, research immune responses, and study immunodeficiencies.
Genscript Corporation CHO-K1/ADRB1/G15 Stable Cell
The -adrenergic receptors are linked to G proteins The -receptor has three known subtypes Beta-1 receptors primarily regulate myocardial tissue and affect the rate of contraction via impulse conduction Beta-2 receptors regulate smooth muscle tone and influence vascular and bronchiolar relaxation Beta-3 receptors are less well studied but are thought to primarily affect lypolysis and may have effects on cardiac inotropy (Greene Shepherd 2006) In the human heart beta (1)- and beta (2)AR are the most powerful physiologic mechanism to acutely increase cardiac performance Changes in betaAR play an important role in chronic heart failure (CHF) Thus due to increased sympathetic activity in CHF betaAR are chronically (over) stimulated and that results in beta (1) AR desensitization and alterations of down-stream mechanisms (Brodde OE 2006)
Eurofins Discoverx PathHunter HEK 293 ADRA1D β-Arrestin Cell line, 2 vials. (93-0983C1)
The PathHunter ADRA1D cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1D GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA1D and EA-tagged β-Arrestin-2. Activation of ADRA1D stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Section - Lupus: Blood Vessel: Artery, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Small Intestine, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx PathHunter U2OS mHTR2C β-Arrestin Cell Line, 2 vials. (93-0790C3)
The PathHunter mHTR2C cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of mHTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mHTR2C and EA-tagged β-Arrestin-2. Activation of mHTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Genscript Corporation CHO-K1/G15 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human G protein subunit alpha 15 (G 15) in the cytoplasm This protein enables G protein-coupled receptor binding activity and acts upstream of or within with a positive effect on calcium-mediated signaling